Monday morning started off nice and morning-like. Now, I am someone who prides herself on her promptness. I try very hard to be early to almost everything and very rarely end up late. However, the last week or so at the Museum the lack of official time I am required to come in has led to late mornings. Generally I shoot for 10am, and generally I make it in by 10:15 but there are occasional slip ups. Monday, however, I had a meeting scheduled with the guy in charge of all the labs in the museum at 10am so I really had to get up, get going, and arrive at the museum on time. I was happy to see that given the requirement of 10am I was back to my usual self, showing up at 9:45 and waiting outside his office for 15 minutes. haha. The walkthrough itself took about 45 minutes. He took me through all the different lab areas, pointing out important features as we went and informing me of any and all potential health hazards. Luckily, it seems as though there aren't too many terribly hazardous chemicals in the lab, so there's really only so much damage I could do if something went wrong. He did inform me however, that a specific fume hood was where they do testing on various birds that are sent to them from the government and sometimes other countries that they test for various very deadly viruses and diseases. Sooooo, lets just say I will be staying as far away from that fume hood as possible. No dead bird infections for me, please :)
After walking through the labs (which luckily I was already fairly familiar with since I had already worked with Andrew in them) we just sat and went over additional safety information and logistical stuff. The guy was extremely nice and I actually really enjoyed the 45-minute run-through. After, I ventured back to the 'old infants school' (the name of the building that Andrew's office is in.... weird, I know, but I guess that's what it used to be wayyyy back when!) The rest of the day I worked in his office taking more photos of specimen and organizing some display boxes to determine which specimen would be used for our next collection. In the afternoon Andrew mentioned that next week we will be discussing and deciding what specimen and project I will be heading during my time here for a paper for me to co-publish with him! He seems very excited about the idea and very determined to make sure I get to have something published, so that's exciting. At the end of the day I packed up and headed out after my tasks were complete. I stopped on my way home to grab a few groceries and once I got home I totally crashed. For some reason I was wholly exhausted and I ended up spending most of the night just chillin'. At the end of the night though I decided to give myself a mani/pedi and it turned out super successfully. I put flowers on them and everything!
From LA to Australia, here are my random ramblings of ridiculousness XD
Monday, March 7, 2011
Sunday, March 6, 2011
Day 15: Lal Qila
Saturday, March 5, 2011
Day 14: MARDI GRAS!!
Friday, March 4, 2011
Day 13: The Invisible Roomie Emerges!
Thursday, March 3, 2011
Day 12: Pictures and Dramzzz
I woke up to a lovely text from Dr. Mitchell telling me to come in at 11 instead of 10, yayy! Another morning of oatmeal, watery chai, and a little bit of good news from some grad schools. When I got to work I discovered that my project would be to organize and photograph about 90 moth specimen to get them ready for more collections. Now, I know taking pictures is fun, but boy-o-boy insect pictures are hard work. It literally took me from 11am till 4pm to photograph all 90 because they require special light, very specific camera settings, and manually focusing each because they're so small and detailed. Manually focusing was probably what sucked the most. I took on average 5 pictures of each specimen and kept the one that was best in focus (meaning the middle of the wing was completely in focus and the rest of the insect was as in focus as possible). It was a long day, but I was proud by the end of it because I was able to finish the whole thing. I took a short break in the middle to eat my packed PBJ sandwich (so yummy, something about the PB here is wayyyy better than in the states) but otherwise I worked through and accomplished the task. I went home with a little bit of blurry vision (lol) but otherwise happy and arrived home to just Anton in the apartment. I was trying to figure out if I should go out, but because of the combination of heat and exhaustion I decided another night in was acceptable. I sat down at the computer and figured out a way to make netflix work for me in AU which made me super super happy. None of my other tv websites work here, so I was getting a little bored since we have 9 channels and AU is about 2 weeks behind the most recent US episode of all the shows. Right as I was settling down to watch a tv ep there was a knock at the door. Anton opened it and I heard a few voices asking if they could come in. I was a bit confused, but figured they must be friends of his or something, but I was wrong. 5 people came inside and immediately announced that they were from immigration and they were looking for someone that they heard from a tip lived here. Now, I just moved in and to be honest was struggling to remember everyone's name so I stayed quiet on the couch. When they got into the living room they asked Anton for his name and asked me for the same. They said they were looking for Jae Thi Li Chan and when the lady looked over to me and asked if he lived here I told her I had no idea (real helpful, right?). Anton also said he didn't know, and because his english isn't as strong the speed and accent with which the main lady talked was a little too much for him I think. They requested to see identification from both of us and while I went to get mine Jonathan came home. I came back in the living room as he was being asked to sit down in one of the computer chairs to show his ID as well. Turns out Jonathan = Jae and I guess he must have overstayed his welcome in AU. They took both Anton and my passports to verify them over the phone and ensure that we were both on valid visa's and apparently Anton has also only been here for like 2 weeks kinda like me. They asked Jonathan to talk to him in his room and he and two of the Immigration people disappeared into his room for almost 30 minutes to talk. When they finally came out he had a bag packed and they basically all left with him. As soon as they were gone Anton and I both looked at each other and simultaneously were like WTF!?!? He said that when they first asked to come in he thought it might be a robbery since they didn't say much, but that the whole situation was crazy strange. Neither of us really knew what to do, and since I just moved in and he moved in a few days before me we just kind of sat there talking about how ridiculous it was and how weird it was that they just kind of took him away. Now, I have no idea what they do with people that have overstayed their visa, nor do I know when/if he'll be returning but I assume they have to let him because he only took a duffle bag worth of stuff and things like his computer and dvds and such are still here. It was a very strange experience to have and I sure hope I never get on the wrong side of immigration people cause they were intense and one of the guys was effing HUGE. A little while after they left Anton said he needed to go on a walk because the whole thing kind of startled him and I decided to try and make some dinner. I ended up making rice with grilled red peppers, carrots, chicken, and soy sauce. By the time I was done making dinner Anton came back and we talked about how awkward it would be to have to tell Willy and Yalin that Jonathan was gone. Luckily for me Anton had known Yalin a little before moving in so he called her and let her know and then had to leave again to get to a study group. The rest of the night I was mostly flabbergasted by the days events so I just chilled, watched a movie or two on netflix and decided to take it easy.
Wednesday, March 2, 2011
Day 11: PCR - no, that doesn't stand for Pretty Cool Reseach, though it could!
Morning two of waking up in my own apartment and it was just as pleasant as the first! I woke up again to see Yalin sleeping in bed after getting back from work at about 5am. I overslept a bit, so I hurried out the door but was still able to make some time for oatmeal and a very watery homemade chai latte. When I got to work Andrew (Dr. Mitchell) said we were starting off the day with the next step of the Barcoding Process, PCRs. PCRs, otherwise known as polymerase chain reactions is a very intricate process that luckily a machine does most of! We went up into a different lab in the super sterile clean area to prepare the samples which basically means adding a bunch of different chemicals, binders, and catalysts to actually sol and replicate the DNA. Once all the proper chemicals are added we put it in a machine to do the actual reactions which involved three major steps: denaturation, annealing, and extension - all done at different temperatures for different lengths of time to produce copies of the DNA. The process takes about 2 hours so while we waited we went out to lunch with the other volunteers in the Entymology Dept. Now, I am by far the youngest volunteer (by at least 20 years) but it was really fun talking with everyone. I was sitting next to a woman that's volunteered for over 5 years and she ended up being a flute tutor! Her granchildren are all in band and her oldest grandson recently traveled to the US with his band to play in a concert which was so cool. I had a slice of banana bread at lunch which was absolutely delish toasted with butter and passed on the lattes everyone seemed to be having because they were ridiculously expensive. When we got back to the lab we retreived the samples from the PCR machine and did what's called a Gel experiment that tests whether or not we got suitable DNA. The test is really pretty and creates kind of a rainbow effect in the otherwise clear gel - it was probably my favorite part of the day. On the way home I stopped by pizza hut to get a $5 pizza mia! I didn't feel much like cooking, and let's be real, who can pass up The Hut?! On my way home I also noticed that there was a HUGE American Express building in downtown and observed that no other major credit card company had a building (that I could see) in downtown Sydney. I found it fairly ironic that of all the credit card companies AMERICAN express was the one doing the best in AU. haha. I went home to enjoy the pizza and once again found myself in the living room with two of the boys while I did some laundry. One of them, Jonathan, saw me sitting on the floor to charge my laptop and actually went and got me an extension cord which was really really nice of him. He also taught me how to use their TV since the remote is tricky and helped me to navigate the kitchen a little better than I was previously. I learned than Anton, the other male roomie is attending UTS in Sydney for engineering and he can actually play the guitar quite well. Jonathan made a little bit of small talk with me which I appreciated, but was mostly into watching The Simpsons on his desktop computer. Mostly they were quiet, sometimes stopping to talk to each other in Chinese, but it was kinda nice just being able to chill out. It's a little strange not having Yalin around in the evenings. On the one hand its awesome basically having my own room, but on the other it could be interesting to see how all these boys warm up to me. LOL. Who knows, maybe we'll all be besties ;)
The Harbor I walk by on the way home.. There was a lightning storm I was trying to snap a pic of, but unfortunately my eyes and my hands didn't move fast enough to snap a pic. To the right of the end of the pic is the Sydney Aquarium and Animal World and to the left is the Maritime Museum :) Nice walk homeeee.
Tuesday, March 1, 2011
Day 10: Well Hello There, Qiacube!
My first full day in my apartment started well! I woke up to get ready for work at about 9 and found that I was the only person awake in the apartment. I took my time getting ready and actually made myself breakfast which is fairly unheard of... Don't worry people, it was only oatmeal! Once I got to work I was excited to see Dr. Mitchell and get working on continuing the barcoding that we had begun on Monday. When I got there in the morning we went straight to work on the 12 samples. We took them out of the heating station we had them baking in at 50'C overnight and headed straight to the lab. I unfortunately committed the faux paux of wearing flip flops on a day that we were heading up to the lab, but Dr. Mitchell said not to worry about it so I guess they aren't too strict around these parts. As we walked up I met the man in charge of the lab area and Dr. Mitchell set up next Monday for me to do my official lab run through with him so I would be able to work through the process alone in order to get as many samples done as possible which made me really happy. I don't know why, but I didn't really think he would trust me with all the lab work alone. I'm just a girl with a degree that doesn't know diddly-squat about DNA Barcoding. He kept using lingo as we went along that I was hard-pressed to keep up with, but he was also good about trying to explain everything thoroughly as we went. In the lab we donned lab coats and gloves and continued the Barcoding process. This basically involved transferring the DNA solution into new test tubes (leaving behind the actual moth matter) and putting them into a machine called the Qiacube. Now this machine is hella legit. Dr. Mitchell thought we were going to have to do all the stuff by hand, but luckily the machine was available and it apparently saved us a lot of time using it. After we headed back to his office and he informed me that they were having a free BBQ for Museum staff for lunch, which was awesome. I had a turkey dog and a beer for lunch (who can say no to a free Australian beer that you've never tried?). I spent the lunch chatting with a couple of the Museum staff and specifically this very interesting woman who does a lot of agricultural research in Papua New Ginneau. It was good stuff. After lunch I worked to photograph the specimen that we were currently testing and called it a day by about 4pm leaving with a print out of a 50 pg. manual Dr. Mitchell wrote for one of his classes on DNA Barcoding, PCRs, and some other stuff I should really bulk up on. I walked home, stopping to buy a few more groceries and necessities I had forgotten to buy earlier at one of the larger markets. When I got home two of the boys were home, but Yalin my roomie wasn't because she works night shifts. So I basically hung out in the living room (that's where the strongest internet signal is), surfed the web, and tried to get a little more acquainted with the apartment. I made some dinner with the rice cooker and worked my way through the manual. It was a little awkward since I had only met the guys once, and since everyone was doing their own thing we didn't talk at all, but I figured that's the way it starts out. So no biggie. I worked my way through the entire manual and thanks to a couple videos that Dr. Mitchell showed me before I left I actually understood most of what it said. After a long hot day I decided to pass out after I finished settling in so I would be ready for another day.
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